Cytobank Inc.

Scientific Expertise:

Our scientific interests and work involve all aspects of flow cytometry, including standard immunophenotyping, fluorescence activated cell sorting (FACS), phospho-flow, multi-dimensional analysis, intracellular staining, and single cell signaling profiles. We have been part of research projects in cancer, biochemistry, virology, and immunology. We are enthusiastic about spreading flow cytometry technologies and are happy to work with you on experiment analysis, data storage and searching, experiment design, and protocol troubleshooting.

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Technical Expertise:

Our technical skills and passions are in building scalable web applications. We have worked with a variety of technologies including Ruby/JRuby on Rails, MySQL, Enterprise Java, Javascript and HAML/SASS. We actively work on Cytobank, a platform to manage share and analyze flow cytometry data over the web.

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A selected list of our publications are below:

Kotecha N, Krutzik PO, Irish JM. Web based analysis and publication of flow cytometry experiments. Current Protocols in Cytometry. July 2010.

Chapter on using Cytobank for managing, sharing and analyzing flow cytometry data over the web.

Irish JM, Hovland R, Krutzik PO, Perez OD, Bruserud Ø, Gjertsen BT, Nolan GP. Single cell profiling of potentiated phospho-protein networks in tumor cells. Cell 2004 Jul 23;118(2):217-28. PMID: 15260991

Created single cell signaling profiles and used this technique to stratify acute myelogenous leukemia patient clinical outcomes.

Krutzik PO, Crane JM, Clutter MR, Nolan GP. High-content single-cell drug screening with phosphospecific flow cytometry. Nat Chem Biol. 2008 Feb;4(2):132-42. PMID: 18157122

A platform based on phosphospecific flow cytometry that enabled screening for inhibitors against multiple endogenous kinase signaling pathways in heterogeneous primary cell populations at the single-cell level.

Kotecha N, Flores NJ, Irish JM, Simonds E, Sakaguchi D, Archambeault S, Diaz-Flores E, Coram M, Shannon KM, Nolan GP, Loh ML. Single cell profiling identifies aberrant STAT5 activation in myeloid malignancies with specific clinical and biologic features. Cancer Cell 2008 Oct 7;14(4):335-43. PMID: 18835035

Transformed the month long diagnostic assay for confirming juvenile myelomonocytic leukemia into a one day signaling assay.

Krutzik PO, Nolan GP. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nat Methods. 2006 May;3(5):361-8. PMID: 16628206

Developed a cell-based multiplexing technique called fluorescent cell barcoding (FCB) to add high-throughput capacity of flow cytometry samples.

Irish JM, Kotecha N, Nolan GP. Mapping normal and cancer cell signalling networks: towards single-cell proteomics. Nature Reviews Cancer 2006 Feb;6(2):146-55. PMID: 16491074

Innovation article describing the novel ‘single cell signaling profile’ approach to dissecting cancer cell signaling networks and stratification of patient outcome.

Hammer MM, Kotecha N, Irish JM, Nolan GP, Krutzik PO. WebFlow: a software package for high-throughput analysis of flow cytometry data. Assay Drug Dev Technol. 2009 Feb;7(1):44-55. PMID: 19187010

Highlights a web-approach for analysis of flow cytometric data from high-throughput drug screening or disease profiling experiments.

For a full list of publications visit PubMed.gov.

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